Measuring the metabolic activities in living organisms is a well established science. In 1784, Antoine Laurent Lavoisier and Pierre Simon de Laplace cleverly devised the first calorimetric device, using heat to measure chemical and physical changes. Calorimeters have evolved to become a modern tool for the advancement of science. SymCel is introducing the first calorimeter developed specifically for cell-based assays suitable for both advanced metabolic research as well as drug discovery and development applications.
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calScreener™ technology is valid for monitoring changes in biological processes caused by physical, chemical or biological stimuli. Changes in metabolic activity will cause changes in heat dissipated from the cell, tissue or organism. Depending on the biological process involved different kinetic behaviors are anticipated. The graphs are idealized examples of the different heat output over time from different cellular processes.
The CalScreener™ principle
Biological processes caused by physical, chemical or biological stimuli in which metabolic changes are anticipated are all valid for the analysis.
The calPlate™ containing the individual sealed cups holding the cell culture are placed in a thermostatic chamber set at the target temperature with a precision within thousands of a Kelvin.
The cups rest upon a heat-flux detecting sensor, the thermopile. The sensor is attached to a heat-sink with a large mass compared to the cell-culture cups. All heat produced is transferred to the heat-sink giving rise to a signal in the thermopile sensor proportional to the heat-flow.
The measured heat is thus independent of the model system or the process involved. We have a label free, real-time, detection system applicable to a wide range of biological applications
Calorimetry technology can be applied to
Bioavailability – Are your compounds able to affect living cells?, Target validation, Hit validation; rapid assessment of effect on cells, Rapid filtering of hit compounds with in-built toxicity testing & Lead selection.
Identification of High-producing Clones & Optimization of Culture Conditions.
Identify toxicological events at early stage in the discovery process.
Metabolic monitoring & Proliferation assays.
calScreener™ is not limited to these few applications. The application areas are limited only by the imagination of the scientist. We strongly encourage you to discuss with us your label-free cell-assay ideas and requirements.
Below are some publication examples of biological processes and applications where heat measurements
have been conducted using calorimetric equipment, including measurement of basic
cellular responses such as cell proliferation, cell death (apoptosis) and cell signaling.
Braissant O, Keiser J, Meister I, Bachmann A, Wirz D, Göpfert B, Bonkat G, Wadsö I. Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label-free well-plate assay. Biotechnol J. 2015 Mar;10(3):460-8. doi: 10.1002/biot.201400494. Epub 2015 Feb 18. PubMed PMID:25511812; PubMed Central PMCID: PMC4406140.
- Flores D, Panic G, Braissant O, Keiser J. A novel isothermal microcalorimetry tool to assess drug effects on Ancylostoma ceylanicum and Necator americanus. Appl Microbiol Biotechnol. 2015 Oct 30. [Epub ahead of print] PubMed PMID: 26519051.
- Bermudez, J., P. Backman, et al. (1992). “Microcalorimetric evaluation of the effects of methotrexate and 6-thioguanine on sensitive T-lymphoma cells and on a methotrexate-resistant subline.” Cell Biophys. 20(2-3): 111-23.
- Wallen-Öhman, M., P. Lönnbro, et al. (1993). “Antibody-induced apoptosis in a human leukemia cell line is energy dependent: thermochemical analysis of cellular metabolism.” Cancer Letters 75(2): 103-9.
- Roig, T. and J. Bermudez (1995). “Microcalorimetric evaluation of the effect of combined chemotherapeutic drugs.” Biochim Biophys Acta. 1244(2-3): 283-90.
- Bluthnerhassler, C., M. Karnebogen, et al. (1995). “Influence of Malignancy and Cyctostatic Treatment on Microcalorimetric Behavior of Urological Tissue Samples and Cell-Cultures.” Thermochimica Acta 251: 145-154.
- Böttcher, H. and P. Fürst (1996). “Microcalorimetric and biochemical investigations of thermogenesis and metabolic pathways in human white adipocytes.” Int J Obes Relat Metab Disord. 20(9): 874-81.
- Hinz, W., B. Faller, et al. (1999). “Recombinant human uncoupling protein-3 increases thermogenesis in yeast cells.” FEBS Lett. 448(1): 57-61.
- Feng, Y., S. F. Luo, et al. (1997). “Study on the thermosensitivity of a tumor cell by microcalorimetry.” Thermochimica Acta 303(2): 203-207.
- Barros, N., S. Feijoo, et al. (2001). “Interpretation of the metabolic enthalpy change, DHmet, calculated for microbial growth reactions in soils.” Journal of Thermal Analysis and Calorimetry 63(2): 577-588.
- Dejean, L., O. Bunoust, et al. (2002). “Control of growth yield of yeast on respiratory substrate by mitochondrial content.” Thermochimica Acta 394(1-2): 113-121.